Most Read

  1. Pneumococcal Vaccine as One of the Immunization Coverage Targets for Adulthood Vaccines: A Consensus Report of the Study Group for Adult Immunization of the Turkish Society of Clinical Microbiology and Infectious Diseases
  2. Does Hepatitis B Affect Erectile Dysfunction and Lower Urinary Tract Symptoms?
  3. HIV Infection in Turkey: How Close Are We to the Target?
  4. Evaluation of HBeAg-Negative Chronic Hepatitis B Patients Who Had Undergone Liver Biopsy in Accordance With the Guidelines
  5. Diagnosis, Treatment and Prevention of Diabetic Foot Wounds and Infections: Turkish Consensus Report
  6. When to Stop Antiviral Therapy in Chronic Hepatitis B
  7. Mycoplasma hominis and Ureaplasma urealyticum: Their Role in Urogenital System Infections and Retrospective Evaluation of Antibiotic Resistance Rates
  8. Crimean-Congo Haemorrhagic Fever: Treatment and Use of Ribavirin
  9. Modern Diagnostic Methods Used in Bacteriology: Rapid and Effective
  10. A Case of Generalized Tetanus With a Short Incubation Period
  11. Analysis of HIV/AIDS-Related Stigma and Discrimination in Turkey: Results of the People Living With HIV Stigma Index
  12. Does Anti-HIV Positivity in Pregnancy Suggest the Requirement of HIV Prophylaxis for Newborn?

Volume 33, Issue 1

Original Article

Confirmatory Test Results in Patients With a Reactive Anti-HIV Test Result: Four-Year Data

Ayşe Nuriye Varışlı
Affiliations
  • Kırıkkale Yüksek İhtisas Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, Kırıkkale, Türkiye
,
Tülin Demir
Affiliations
  • Halk Sağlığı Genel Müdürlüğü, Mikrobiyoloji Referans Laboratuvarları ve Biyolojik Ürünler Daire Başkanlığı, Ulusal HIV-AIDS Doğrulama ve Viral Hepatitler Referans Laboratuvarı, Ankara, Türkiye
,
Süda Tekin
Affiliations
  • Koç Üniversitesi Hastanesi, İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, İstanbul, Türkiye
  • AntiHIV 12 reactivity
  • confirmatory test

Objective: The aim of this study was to evaluate the results of confirmatory tests performed by line immunoassay (LIA) in blood donors and patients with suspected HIV infection, and patients undergoing preoperative or antenatal screening, of whom anti-HIV-1/2 test results were found as repeatedly reactive.

Methods: Serum samples sent to the microbiology laboratory between January 2014 and December 2017, were tested by the fourth-generation enzyme-linked immunosorbent assay (ELISA), which identifies the anti-HIV-1/2 antibodies and p24 antigen together. The repeatedly reactive samples were investigated by LIA method as a confirmatory test.

Results: A total of 106 786 patients’sera were screened by ELISA. Of these patients, 47 723 (45%) were male and 59 063 (55%) were female. The distribution included 37 375 (35%) patients from inpatient clinic and intensive care unit, 2040 (2%) blood donors, 1023 (1%) dialysis patients, 4271 (4%) pregnant women, 2135 (2%) patients from infectious diseases department and 59 942 (56%) patients from other outpatient clinics. In sera of 90 (%0.08) patients aged 15-93 years, the HIV1-2 antibody was found as reactive by ELISA. 14 (15.9%) positive, 4 (4.5%) indeterminate and 70 (79.5%) negative results were found in samples investigated by LIA. Two samples which were not repeatedly reactive with ELISA were not tested by the confirmatory test. Confirmed HIV-positive cases over the years were distributed as 3 (21%) in 2014, 2 (14%) in 2015, 4 (29%) in 2016, and 5 (36%) in 2017, and the increase in cases according to years was found statistically significant (p<0.001).

Conclusions: In our study, anti-HIV detection rates in patients were found to be consistent with other studies conducted on this subject.79.5% of patients with reactive ELISA and anti-HIV 1-2 tests were considered false-positive. These patients had autoimmune diseases, malignancy, pregnancy, and endocrinological disorders. As a result, HIV positivity among patients investigated by confirmatory tests has increased in recent years.

Klimik Dergisi 2020; 33(1): 24-8.

Cite this article as: Varışlı AN, Demir T, Tekin S. [Confirmatory test results in patients with a reactive anti-HIV test result: Four-year data]. Klimik Derg. 2020; 33(1): 24-8. Turkish.

  • 24-28
  • 10.5152/kd.2020.05
  • April 29, 2020
  • 1977
  • 685
Download as PDF
  • This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. CC BY-NC
Volume 37, Issue 3 Volume 37, Issue 2 Volume 37, Issue 1 Volume 36, Issue 4 Volume 36, Supplement 1 Volume 36, Issue 3 Volume 36, Issue 2 Volume 36, Issue 1 Volume 35, Issue 4 Volume 35, Issue 3 Volume 35, Issue 2 Volume 35, Issue 1 Volume 34, Issue 3 Volume 34, Issue 2 Volume 34, Issue 1 Volume 33, Issue 3 Volume 33, Issue 2 Volume 33, Issue 1 Volume 32, Issue 3 Volume 32, Supplement 1 Volume 32, Supplement 2 Volume 32, Issue 2 Volume 32, Issue 1 Volume 31, Issue 3 Volume 31, Issue 2 Volume 31, Supplement 1 Volume 31, Issue 1 Volume 30, Issue 3 Volume 30, Issue 2 Volume 30, Supplement 1 Volume 30, Issue 1 Volume 29, Issue 3 Volume 29, Issue 2 Volume 29, Issue 1 Volume 28, Supplement 1 Volume 28, Issue 3 Volume 28, Issue 2 Volume 28, Issue 1 Volume 27, Supplement 1 Volume 27, Issue 3 Volume 27, Issue 2 Volume 27, Issue 1 Volume 26, Issue 3 Volume 26, Supplement 1 Volume 26, Issue 2 Volume 26, Issue 1 Volume 25, Issue 3 Volume 25, Issue 2 Volume 25, Issue 1 Volume 24, Issue 3 Volume 24, Issue 2 Volume 24, Issue 1 Volume 23, Issue 3 Volume 23, Issue 2 Volume 23, Issue 1 Volume 22, Issue 3 Volume 22, Issue 2 Volume 22, Issue 1 Volume 21, Issue 3 Volume 21, Supplement 2 Volume 21, Supplement 1 Volume 21, Issue 2 Volume 21, Issue 1 Volume 20, Issue 3 Volume 20, Supplement 2 Volume 20, Issue 2 Volume 20, Issue 1 Volume 20, Supplement 1 Volume 19, Issue 3 Volume 19, Issue 2 Volume 19, Issue 1 Volume 18, Issue 3 Volume 18, Supplement 1 Volume 18, Issue 2 Volume 18, Issue 1 Volume 17, Issue 3 Volume 17, Issue 2 Volume 17, Issue 1 Volume 16, Issue 3 Volume 16, Issue 2 Volume 16, Issue 1 Volume 1, Supplement 1 Volume 15, Issue 3 Volume 15, Issue 2 Volume 15, Issue 1 Volume 14, Issue 3 Volume 14, Issue 2 Volume 14, Issue 1 Volume 13, Issue 3 Volume 13, Issue 2 Volume 13, Supplement 1 Volume 13, Issue 1 Volume 12, Issue 3 Volume 12, Issue 2 Volume 12, Issue 1 Volume 11, Issue 3 Volume 11, Issue 2 Volume 11, Supplement 1 Volume 11, Issue 1 Volume 10, Issue 3 Volume 10, Issue 2 Volume 10, Issue 1 Volume 9, Issue 3 Volume 9, Issue 2 Volume 9, Issue 1 Volume 8, Issue 3 Volume 8, Issue 2 Volume 8, Issue 1 Volume 6, Issue 3 Volume 7, Issue 1 Volume 7, Issue 2 Volume 7, Issue 3 Volume 4, Issue 3 Volume 5, Issue 1 Volume 5, Issue 2 Volume 5, Issue 3 Volume 6, Issue 1 Volume 6, Issue 2 Volume 3, Issue 1 Volume 3, Issue 2 Volume 3, Issue 3 Volume 4, Issue 1 Volume 4, Issue 2 Volume 1, Issue 2 Volume 2, Issue 1 Volume 2, Issue 2 Volume 2, Issue 3 Volume 1, Issue 1