Abstract

Sepsis is one of the leading causes of morbidity and mortality due to infections. Traditional blood culture methods have long been accepted as the gold standard reference method for diagnosing sepsis. However, conventional methods have many limitations, such as the long identification period of pathogenic microorganisms, low sensitivity, and the need for frequent repetition. Therefore, molecular methods, sequencing technologies, nanoparticles, microfluidic systems, biosensors, and artificial intelligence algorithms have been used to diagnose of sepsis. This review will discuss molecular techniques for rapidly identifying the causative microorganism, especially on whole blood samples, and commercial tests developed based on these molecular methods.