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Volume 38, Issue 1

Original Article

Interpretation of Molecular Panel Results in the Diagnosis of Respiratory Tract Infections According to Clinical and Demographic Features

Mediha Uğur
Affiliations
  • Giresun Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Giresun, Türkiye
https://orcid.org/0000-0002-2526-397X
,
Ahmet Melih Şahin
Affiliations
  • Giresun Üniversitesi Tıp Fakültesi, Klinik Mikrobiyoloji ve İnfeksiyon Hastalıkları Anabilim Dalı, Giresun, Türkiye
https://orcid.org/0000-0003-3670-7801
,
Esin Avcı
Affiliations
  • Giresun Üniversitesi Fen Edebiyat Fakültesi, İstatistik Bölümü, Giresun, Türkiye
https://orcid.org/0000-0002-9173-0142
  • molecular methods
  • respiratory panel
  • respiratory tract infections

Abstract

Objective: Respiratory tract infections are a significant public health problem worldwide. The molecular-based respiratory panels currently in use can identify pathogens with high sensitivity in a short period of time. Our study aims to contribute to the rational use of the test by determining the relationship between test positivity and the patient’s demographic data, symptoms and complaints, comorbidities, and initial laboratory parameters.

Methods: Between September 2023 and April 2024, 915 samples with respiratory panel requests were included in the study. The samples were studied with the Qiagen® QIAstat-Dx Analyzer 1.0 (Qiagen, Germany) and QIAstat-Dx® Respiratory SARS-CoV-2 Panel Kit (Qiagen, Germany). The information about clinical departments from which the samples were sent, the patients’ epidemiological characteristics, complaints at the time of the request, leukocyte and C-reactive protein (CRP) values, and comorbidities during that period were obtained retrospectively from patient files. 

Results: Out of the 915 samples, 559 (61.09%) tested positive. There was no significant difference between test-positive and negative patients regarding gender and the hospital department where patients were admitted. A statistically significant difference was observed in positivity rates of patients with cough, nasal discharge, sputum, and sore throat, and in the group under 18 years of age (p<0.05). The presence of comorbidities was statistically significant in the panel-negative group (p<0.05). A reliable distinctive cut-off point was not determined for CRP and leukocyte values in the receiver operating characteristic curve (ROC).  Cough, age, comorbidities, and dyspnea were found to be factors influencing the panel results according to the Chi-square Automatic Interaction Detection (CHAID) algorithm.

Conclusion:  Rational use of the panel will allow rapid diagnosis and early initiation of appropriate treatment in the presence of respiratory symptoms and will reduce the economic burden.

Show References

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  • 36-44
  • Mediha Uğur
  • medihacerrah@hotmail.com
  • 10.36519/kd.2025.5162
  • November 15, 2024
  • February 18, 2025
  • March 21, 2025

  • Uğur M, Şahin AM, Avcı E. [Interpretation of molecular panel results in the diagnosis of respiratory tract infections according to clinical and demographic features]. Klimik Derg. 2025;37(4):36-44. Turkish.
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