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Volume 31, Issue 1

Original Article

Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment

Mehalene Jayaram
Affiliations
  • School of Biological Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, Malaysia
,
Hideyuki Nagao
Affiliations
  • School of Biological Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang, Malaysia
  • Black yeast
  • environment
  • Exophiala dermatitidis
  • pigeon
  • roseBengal

Abstract

Objective: The objective of this study is to describe the potato dextrose agar (PDA) with rose-Bengal and chloramphenicol as a new and simple medium (R-PDA chloramphenicol agar) to facilitate the detection of black yeast, Exophiala dermatitidis compared to ready-made conventional medium, namely rose-Bengal chloramphenicol agar.

 

Methods: We prepared a new medium by adding chloramphenicol and rose-Bengal to ready-made PDA.

 

Results: This medium proves better growth of the black yeast in terms of increased colony size compared to commercial rose-Bengal chloramphenicol agar. The increase in colony size aids for distinguishing the slow growing black yeast from all the other filamentous fungi.

 

Conclusions: Compared to traditional rose-Bengal chloramphenicol agar, R-PDA chloramphenicol agar is superior to isolate Exophiala dermatitidis among other fast growing filamentous fungi which are present in the environment samples.

 

Cite this article as: Jayaram M, Nagao H. Potato dextrose agar with rose-Bengal and chloramphenicol: A new culture medium to isolate pathogenic Exophiala dermatitidis from the environment. Klimik Derg. 2018; 31(1): 11-5.

  • 11-15
  • 10.5152/kd.2018.05
  • July 23, 2019
  • 4485
  • 1062
Download as PDF
  • This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. CC BY-NC
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